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タイトル :The Effect of Pulsed Streamer-like Discharge in Liquid on Transcriptional Activation of Retrotransposon Genes of a Red Alga, Porphyra Yezoensis
著者 :Ohno, T.
Li, Z.
Lin, X.F.
Zhang, W.B.
Takano, H.
Takio, S.
Namihira, T.
Akiyama, H.
刊行年月日 :2007-6
収録雑誌名 :Digest of Technical Papers-IEEE International Pulsed Power Conference
巻 :2007
開始ページ :403
終了ページ :406
要約(Abstract) :Retrotransposons are mobile genetic elements that accomplished transposition via an RNA intermediate. These elements can be transcriptionally activated by stress factors, such as UV light, ozone, pathogens, wounding and drought. A red alga, porphyra yezoensis has recently been recognized as a model plant for fundamental and applied study in marine biological science. In this paper, pulsed streamer-like discharge in liquid was used as a new stress condition, and the transcription level of a copia-like retrotansposon gene PyRE1 of a red alga, porphyra yezoensis was investigated. From the results of reverse transcription-polymerase chain reaction (RT-PCR) experiments, the transcription of PyRE1 was activated by applying pulsed streamer-like discharge. The results suggest that pulsed streamer-like discharge in liquid can be used as a new stress condition to activate retrotransposon genes and can provide a useful tool for insertion mutagenesis and genome research in biology field.
収録種別 :会議発表論文
出版社(者) :Institute of Electrical and Electronics Engineers
権利(Rights) :(c)2007 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
備考 :16th IEEE International Pulsed Power Conference, Albuquerque, New Mexico, June 17-22, 2007
URI :http://hdl.handle.net/2298/10312
このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/2298/10312