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Title :Cessation of gastrulation is mediated by suppression of epithelial-mesenchymal transition at the ventral ectodermal ridge
Authors :Ohta, Sho
Suzuki, Kentaro
Tachibana, Katsuro
Tanaka Hideaki
Yamada, Gen
Issue Date :Dec-2007
Citation jtitle :Development
vol. :134
no. :24
start page :4315
end page :4324
Abstract :In the gastrula stage embryo, the epiblast migrates toward the primitive streak and ingresses through the primitive groove. Subsequently, the ingressing epiblast cells undergo epithelial-mesenchymal transition (EMT) and differentiate into the definitive endoderm and mesoderm during gastrulation. However, the developmental mechanisms at the end of gastrulation have not yet been elucidated. Histological and genetic analyses of the ventral ectodermal ridge (VER), a derivative of the primitive streak, were performed using chick and mouse embryos. The analyses showed a continued cell movement resembling gastrulation associated with EMT during the early tailbud stage of both embryos. Such gastrulation-like cell movement was gradually attenuated by the absence of EMT during tail development. The kinetics of the expression pattern of noggin (Nog) and basal membrane degradation adjacent to the chick and the mouse VER indicated a correlation between the temporal and/or spatial expression of Nog and the presence of EMT in the VER. Furthermore, Nog overexpression suppressed EMT and arrested ingressive cell movement in the chick VER. Mice mutant in noggin displayed dysregulation of EMT with continued ingressive cell movement. These indicate that the inhibition of Bmp signaling by temporal and/or spatial Nog expression suppresses EMT and leads to the cessation of the ingressive cell movement from the VER at the end of gastrulation.
URL :http://dev.biologists.org/cgi/content/full/134/24/4315
Type Local :雑誌掲載論文
Publisher :Company of Biologists
URI :http://hdl.handle.net/2298/10388
Appears in Collections:Journal Article (Institute of Resource Development and Genetics)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2298/10388