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タイトル :Differentiation of mouse and human ES cells into hepatic lineages
著者 :Shiraki, Nobuaki
Umeda, Kahoko
Sakashita, Naomi
Takeya, Motohiro
Kume, Kazuhiko
Kume, Shoen
刊行年月日 :2008-7
収録雑誌名 :Genes to cells
巻 :13
号 :7
開始ページ :731
終了ページ :746
要約(Abstract) :We recently reported a novel method to induce embryonic stem (ES) cells differentiate into an endodermal fate, especially pancreatic, using a supporting cell line. Here we describe the modified culture condition with the addition and withdrawal of secreted growth factors could induce ES cells to selectively differentiate into a hepatic fate efficiently. The signaling of BMP and FGF that have been implicated in hepatic differentiation during normal embryonic development are shown to play pivotal roles in generating hepatic cells from the definitive endoderm derived from ES cells. Moreover, the expression of AFP, Albumin or a biliary molecular marker appeared sequentially thus suggested the differentiation of ES cells recapitulated normal developmental processes of liver. The ES cell-derived differentiated cells showed evidence of glycogen storage, secreted Albumin, exhibited drug metabolism activities and expressed a set of cytochrome or drug conjugate enzymes, drug transporters specifically expressed in mature hepatocytes. With the same procedure, human ES cells also gave rise to cells with mature hepatocytes’ characteristics. In conclusion, this novel procedure for hepatic differentiation will be useful for elucidation of molecular mechanisms of hepatic fate decision at gut regionalization, and could represent an attractive approach for a surrogate cell source for pharmaceutical studies such as toxicology.
収録種別 :雑誌掲載論文
出版社(者) :Blackwell Publishing
権利(Rights) :© 2008 by the Molecular Biology Society of Japan/Blackwell Publishing
URI :http://hdl.handle.net/2298/11006
出現コレクション:発表論文(発生研)
このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/2298/11006

 

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