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Title :One-step preparation of S-nitrosated human serum albumin with high biological activities
Authors :Ishima, Yu
Hiroyama, Shuichi
Ulrich, Kragh-Hansen
Maruyama, Toru
Sawa, Tomohiro
Akaike, Takaaki
Kai, Toshiya
Otagiri, Masaki
Issue Date :15-Sep-2010
Citation jtitle :Nitric Oxide
vol. :23
no. :2
start page :121
end page :127
Abstract :S-Nitrosated human serum albumin (SNO-HSA) is a large molecular weight nitric oxide carrier in human plasma, and because of its many beneficial effects in different tests, it is currently under investigation as a cytoprotective agent. However, making SNO-HSA preparations is a complicated and time-consuming process. We found that binding of caprylic acid (CA) and N-acetyl-L-tryptophan (N-AcTrp) to defatted mercaptalbumin increased S-nitrosation by S-nitrosoglutathione (GS-NO) by making Cys-34 of HSA more accessible and by protecting it against oxidation, respectively. Fortunately, HSA solutions for clinical use contain high concentrations of CA and N-AcTrp as stabilizers. By making use of that fact it was possible to work-out a fast and simple procedure for producing SNO-HSA: Incubation of a commercial HSA formulation with GS-NO for only one minute results in S-nitrosation of HSA. The biological usefulness of such a preparation was tested in a rat ischemia-reperfusion liver injury model. Although our procedure for making SNO-HSA is fast and straightforward, the cytoprotective effect of the preparation was similar to, or better than, that of a preparation made in a more traditional way. The clinical development of SNO-HSA as a strong cytoprotective agent is under way using this method in collaboration with clinicians and industrial developers.
URL :http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WNT-5017HTP-1&_user=4545557&_coverDate=09%2F15%2F2010&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000008298&_version=1&_urlVersion=0&_userid=4545557&md5=50f652d134dd09a6f72481d07de2387d&searchtype=a
Type Local :雑誌掲載論文
Publisher :Elsevier
Rights :© 2010 Elsevier Inc
URI :http://hdl.handle.net/2298/18631
Appears in Collections:Journal Article (Pharmacy)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2298/18631