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Title :Identification of four sumo paralogs in the medaka fish, oryzias latipes, and their classification into two subfamilies
Authors :Seki, Daisuke
Obata, Shinpei
Shirozu, Tsuyoshi
Kitano, Takeshi
Saitoh, Hisato
Issue Date :Oct-2010
Citation jtitle :Biochemical Genetics
vol. :48
no. :9-10
start page :737
end page :750
Abstract :At least four paralogs of the small ubiquitin-related modifier (SUMO) exist in humans, but there is limited information about SUMO paralogs from other vertebrate species. We isolated the four cDNA encoding proteins, similar to human SUMOs, from the medaka fish, Oryzias latipes: OlSUMO-1, OlSUMO-2, OlSUMO-3, and OlSUMO-4. The amino acid sequences of OlSUMO-2, OlSUMO-3 and OlSUMO-4 are 89‒94% identical, but they share only 45% identity with OlSUMO-1. Phylogenetic analysis, transient expression of OlSUMOs in cultured cells and in vitro binding of OlSUMOs with two different SUMO-interacting proteins demonstrated that the medaka SUMO paralogs can be grouped into two subfamilies, OlSUMO-1 and OlSUMO-2/3/4, respectively. Furthermore, this is the first report of all four OlSUMO transcripts being expressed in medaka embryos, implying that they have a role in fish development. The study will improve understanding of the relationship between structural and functional diversity of SUMO paralogs during vertebrate evolution.
URL :http://www.springerlink.com/content/p7h80l28340t5271/
Type Local :雑誌掲載論文
ISSN :00062928
Publisher :springer
Rights :©Springer Science+Business Media, LLC 2010
URI :http://hdl.handle.net/2298/22281
Appears in Collections:Journal Article (Sciende)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2298/22281