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Title :Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila
Authors :Takahama, Kazuhiro
Tomita, Jun
Ueno, Taro
Yamazaki, Masako
Kume, Shoen
Kume, Kazuhiko
Issue Date :13-Jan-2012
Citation jtitle :Biochemical and Biophysical Research Communications
vol. :417
no. :2
start page :807
end page :811
Abstract :Sleep is a unique behavioral state that is conserved between species, and sleep regulation is closely associated to metabolism and aging. The fruit fly, Drosophila melanogaster has been used to study the molecular mechanism underlying these physiological processes. Here we show that the c-Jun N-terminal Kinase (JNK) gene, known as basket (bsk) in Drosophila, functions in neurons to regulate both sleep and longevity in Drosophila. Pan-neuronal knockdown of JNK mRNA expression by RNA interference resulted in a decrease in both sleep and longevity. A heterozygous knockout of JNK showed similar effects, indicating the molecular specificity. The JNK knockdown showed a normal arousal threshold and sleep rebound, suggesting that the basic sleep mechanism was not affected. JNK is known to be involved in the insulin pathway, which regulates metabolism and longevity. A JNK knockdown in insulin-producing neurons in the pars intercerebralis had slight effects on sleep. However, knocking down JNK in the mushroom body had a significant effect on sleep. These data suggest a unique sleep regulating pathway for JNK.
URL :http://www.sciencedirect.com/science/article/pii/S0006291X11022492
Type Local :雑誌掲載論文
ISSN :0006291X
Publisher :Elsevier
Academic Press Inc
Rights :© 2012 Elsevier
URI :http://hdl.handle.net/2298/24801
Appears in Collections:Journal Article (Institute of Molecular Embryology and Genetics)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2298/24801