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Title :Therapeutic effects of γ-irradiation in a primary effusion lymphoma mouse model
Authors :Shiraishi, Yoshioki
Gotoh, Kumiko
Towata, Tomomi
Shimasaki, Tatsuya
Suzu, Shinya
Kojima, Akihiro
Okada, Seiji
Issue Date :Jan-2010
Citation jtitle :EXPERIMENTAL AND THERAPEUTIC MEDICINE
vol. :1
no. :1(January-February 2010)
start page :79
end page :84
Abstract :Primary effusion lymphoma (PEL) is a unique and recently identified non-Hodgkin's lymphoma in immuno-compromised individuals. PEL is caused by the Kaposi sarcoma-associated herpes virus/human herpes virus 8 (KSHV/HHV-8) and has a peculiar presentation involving liquid growth in the serous body cavity, chemotherapy resistance and poor prognosis. In search of a new therapeutic modality for PEL, we examined the effect of γ-irradiation on PEL-derived cell lines (BCBL-1, BC-1, and BC-3) in vitro and in vivo. An MTT assay and trypan blue exclusion assay revealed that irradiation significantly suppressed cell proliferation in the PEL cell lines in a dose-dependent manner, and induced apoptosis. The PEL cell lines were relatively radiosensitive compared with other hematological tumor cell lines (Raji, Jurkat, and K562 cells). Inoculation of the BC‑3 cell line into the peritoneal cavity of Rag2/Jak3 double-deficient mice led to massive ascites formation, and subcutaneous injection of BCBL-1 led to solid lymphoma formation. Total body irradiation (4 Gy x 2) with bone marrow transplantation resulted in the complete recovery of both types of PEL-inoculated mice. These results suggest that total body irradiation with bone marrow transplantation can be successfully applied for the treatment of chemotherapyresistant PEL.
URL :https://www.spandidos-publications.com/etm/1/1/79
Type Local :雑誌掲載論文
Publisher :Spandidos Publications
URI :http://hdl.handle.net/2298/36143
Appears in Collections:Journal Article (Center for AIDS Research)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2298/36143