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タイトル :Response of biological cell exposed on burst RF fields
著者 :Nomura, Naoyuki
Koga, Y.
Abe, S.
Uchida, Ichiro
Abe, K.
Katsuki, Sunao
Namihira, Takao
Akiyama, Hidenori
Takano, H.
Abe, Shin-Ichi
Kohno, S.
刊行年月日 :2005-6
収録雑誌名 :Digest of Technical Papers-IEEE International Pulsed Power Conference
巻 :2005
開始ページ :1429
終了ページ :1432
要約(Abstract) :Burst RF fields (BRFFs) were applied to Chinese Hamster Ovary (CHO) cells, and the cell response to the BRFF of two different frequencies, 20 kHz and 50 MHz, was investigated by means of the fluorescent microscopy. The field strength and duration of the BRFF were fixed at 1 kV/cm, and 100 μs, respectively. Cells were placed in a 100 μm-gap electrode plated on the slide glass for a microscopy. Acridine orange (AO), which mainly reacts to DNA, was used as the fluorescent dye. From the experiment, the BRFF of 20 kHz initiates the increase in the permeability of cell membrane. In contrast, the BRFF of 50 MHz does not act on cell membrane but causes degeneration of DNA or RNA. We have experimentally demonstrated that the burst RF fields with only a small electric field of 1 kV/cm cause not only membrane reaction but also intracellular effects.
収録種別 :会議発表論文
出版社(者) :Institute of Electrical and Electronics Engineers
権利(Rights) :(c)2005 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
備考 :15th IEEE International Pulsed Power Conference, Monterey, California USA, June 13-17, 2005
URI :http://hdl.handle.net/2298/9720
このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/2298/9720